Analysis Note

Positive ControlHeLa cells, Human esophagus tissue

Application

ELISA (see comments)Immunoblotting (1-5 g/ml)Immunocytochemistry (10 g/ml)Paraffin Sections (6 g/ml)

General description

Purified mouse monoclonal antibody. Recognizes the ~90 kDa LEPRE1 protein.

Recognizes the ~90 kDa LEPRE1 protein in HeLa cells and human esophagus tissue.

This Anti-LEPRE1 Mouse mAb (3C7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of LEPRE1.

Immunogen

A recombinant polypeptide corresponding to amino acids 1-100 of human LEPRE1, expressed as a GST fusion protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Choi, JW, et al. 2009. PLoS Genet.5, e1000750.Cabral, W,A., et al. 2007. Nat Genet.39, 359.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)